Composite

Part:BBa_K3868098:Design

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)


pTargetS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 182
    Illegal EcoRI site found at 552
    Illegal XbaI site found at 188
    Illegal XbaI site found at 558
    Illegal SpeI site found at 73
    Illegal SpeI site found at 443
    Illegal PstI site found at 200
    Illegal PstI site found at 570
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 182
    Illegal EcoRI site found at 552
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal NheI site found at 377
    Illegal NheI site found at 400
    Illegal NheI site found at 420
    Illegal SpeI site found at 73
    Illegal SpeI site found at 443
    Illegal PstI site found at 200
    Illegal PstI site found at 570
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 182
    Illegal EcoRI site found at 552
    Illegal BglII site found at 212
    Illegal BglII site found at 582
    Illegal XhoI site found at 236
    Illegal XhoI site found at 606
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 182
    Illegal EcoRI site found at 552
    Illegal XbaI site found at 188
    Illegal XbaI site found at 558
    Illegal SpeI site found at 73
    Illegal SpeI site found at 443
    Illegal PstI site found at 200
    Illegal PstI site found at 570
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 182
    Illegal EcoRI site found at 552
    Illegal XbaI site found at 188
    Illegal XbaI site found at 558
    Illegal SpeI site found at 73
    Illegal SpeI site found at 443
    Illegal PstI site found at 200
    Illegal PstI site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Gene is from S. pyogenes for the pTargets plasmid of the Crispr-Cas9 system.

References